Cell Culture Process Development and Optimization

Cell culture process development, optimization, and scaling are critical steps in the process of producing biopharmaceutical therapeutics, such as monoclonal antibodies. Continuous in-line real-time measurements can ensure quality, improve efficiency, and increase profits compared to off-line testing. However, in-line real-time measurements can be challenging to obtain with enough sensitivity and accuracy to be useful. The superior performance of Tornado’s Raman Analyzers can help overcome these limitations, making it an ideal solution for cell culture development monitoring in production and in development. Learn how Tornado’s Raman Analyzers are compatible with Sartorius Ambr® and STR® platforms through seamless integration with SpectroPortTM Raman Probe.

Monitoring and Controlling Upstream Bioprocesses

Mammalian Cell culture technology is now considered a robust technique for the manufacturing of many biotherapeutics including monoclonal antibodies. There are a variety of biotherapeutics that are produced in small-scale to large-scale manufacturing facilities for academic interests, for clinical studies, or by biopharmaceutical companies for profit.

Raman spectroscopy has been shown to be able to monitor several important metabolites within mammalian cell cultures and thus provide an important means of control for bioprocesses

Particle Manipulation for Increased Sensitivity and Selectivity in a Cell Reactor

The coupling of the ultrasound technique with Tornado’s Raman spectroscopy allows for in situ sample manipulation to obtain critical information specific to the solid fraction of the cell reactor, i.e. the microorganisms. By utilizing ultrasonic manipulation, particles of the solid fraction can be sequestered by the standing wave in the focal plane of the immersion Raman probe. Trapping of the particles of interest combined with the increased signal strength of the HTVS™ allows for enhanced sensitivity and selectivity in the measurement. Furthermore, additional gains in Raman spectrum quality and sensitivity can be achieved via ultrasonic manipulation of particulates in the sample matrix, as described in this white paper.

Continuous In-Line Control of Perfusion Cell Cultivations

Raman spectroscopy has proven its advantages for process monitoring and control batch cultivations. There is high potential of Tornado’s Raman spectroscopy for advanced process monitoring and control of a perfusion process with a bioreactor and scale-independent measurement method. Tornado’s Raman analyzers with revolutionary spectrometer technology can be used as a PAT tools for in-line glucose control in prolonged perfusion processes, even if low and stable glucose concentrations are required.

Value-Added Means of Accurately Monitoring and Controlling Upstream Bioprocesses

Case Study

The coupling of the ultrasound technique with Tornado’s Raman spectroscopy analyzer allows for in situ sample manipulation to obtain critical information specific to the solid fraction of the cell reactor, i.e. the microorganisms. By utilizing ultrasonic manipulation, particles of the solid fraction can be sequestered by the standing wave in the focal plane of the immersion Raman probe. Trapping of the particles of interest combined with the increased signal strength of the HTVS™ allows for enhanced sensitivity and selectivity in the measurement.

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“Tornado Spectral Systems’ Raman solutions enable applications, that were just not doable before.”

What our
clients say

– Sebastian, Analyticon Instruments GMBH