Real-time process analysis for your bioprocessing needs

Raman spectroscopy is a recognized PAT (Process Analytical Tool) that has been seen as a central spectroscopic tool used in bioprocessing. It plays a pivotal role in the monitoring of fermentations, particularly their optimization within the PAT framework and harnesses biological processes for the development of drug therapies, providing treatments that range from small molecule antibiotics to large recombinant proteins.

Despite the significant growth of Raman spectroscopy in bioprocessing, users still face many challenges. Among these is the need to collect and consolidate data from differing sources to build models at scale.

Bio Processing lab equipment

Thanks to our collaborators at Sartorius, the game has changed. BioPAT® Spectro embraces Quality by Design (QbD) principles and unlocks the potential of Raman spectroscopy. BioPAT® Spectro is compatible with Tornado’s HyperFlux™ PRO Plus Raman analyzer. Consisting of a multi-use flow cell integrated into the Ambr® analysis module, BioPAT® Spectro in Ambr® has been successfully demonstrated by major pharmaceutical companies.

Raman Spectroscopy as a PAT tool for pharmaceutical manufacturing and bioprocessing

BioPAT® Spectro

Tornado HyperFlux™ Raman Bioprocess Solution for High-Throughput Screening and Single-use Bioreactors

Full Integration with Sartorius Ambr® and STR® Platforms

Tornado’s HyperFlux™ PRO Plus and SpectroPort™ Raman probe integrate seamlessly into the Sartorius Ambr® 15 and Ambr® 250 Analysis Modules for quick and simple sampling without the need for pre-sterilization. The PRO Plus Raman Analyzer measures key process parameters and other metabolic species, making it the ideal solution for monitoring bioprocesses and cell cultures.

To learn more, please visit the BioPAT® Spectro page.

Applications & Advantages of Raman Analysis:

  • Monitoring biological processes such as fermentation
  • Monitoring constituents such as nutrient uptake and target molecule production and metabolic indicators
  • Determine end points
  • Optimize equipment turnover
  • Facilitate scale-up
  • Optimize continuous processing
  • Eliminate less efficient monitoring techniques such as HPLC
  • Facilitate QbD
  • Helps meet Right First Time and Zero Defects initiatives

Contact a Tornado expert to discuss your application:


Rapid Development of a 9 component Multivariate Calibration for the analysis of Metabolites in Chemically Defined Cell Culture Media

An assessment by Sanofi UK of the performance of the HyperFlux™ PRO Plus Raman Spectroscopy System for the quantitative analysis of biochemical components in a simplified chemically defined pseudo growth medium for mammalian cell culture.